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BioSyn

All Calculators

Every BioSyn calculator in one directory, grouped by the branch of lab science it serves. All 33 tools are free, run locally in your browser, and show their formula, a worked example and the sources behind it — nothing you enter is uploaded or stored. Start from what you have at the bench, or jump to a category hub below.

Laboratory Calculators

  • C1V1 CalculatorSolve C1V1 = C2V2 for any unknown — stock volume, final concentration or volume. Mix M, mM and µM with L, mL and µL safely and get the diluent volume too.
  • Dilution CalculatorCalculate final concentration, dilution factor or stock and diluent volumes for a 1:X dilution. Works with M, %, mg/mL or CFU/mL, with worked examples.
  • Molar Mass CalculatorCalculate molar mass (g/mol) from any chemical formula — NaCl, H2SO4, C6H12O6 or hydrates like CuSO4·5H2O. Shows element counts and percent composition.
  • Molarity CalculatorCalculate molarity, mass, volume or moles with this free molarity calculator. Shows the M = m/(MW x V) formula, unit conversions and worked lab examples.
  • Percent Solution CalculatorWork out the solute mass or stock volume for % w/v, % v/v and % w/w solutions from a target percentage and final volume or mass, with optional molarity.
  • PPM CalculatorConvert between ppm, mg/L, µg/mL and percent, or work out the solute mass for a target ppm in a given volume, with optional molarity from molecular weight.
  • Serial Dilution CalculatorCalculate the concentration after each step of a serial dilution, the overall factor, or how many 1:X steps reach a target, with per-tube transfer volumes.
  • Stock Solution CalculatorCalculate how many grams of solute to weigh for a molar or % w/v stock solution, with purity correction and step-by-step prep. Free, with worked examples.

DNA & RNA Calculators

  • DNA Concentration CalculatorConvert A260 absorbance to DNA or RNA concentration in µg/mL, mg/mL and nM, with dilution factor, path length and dsDNA/ssDNA/RNA extinction factors.
  • DNA Molecular Weight CalculatorCalculate DNA molecular weight from a sequence or a length, single- or double-stranded, with µg/nmol and nmol/µg conversions and free-acid residue masses.
  • GC Content CalculatorCalculate the GC content of a DNA sequence — GC%, AT%, length and base counts, with IUPAC degenerate-base handling and a low/typical/high call for primers.

PCR & qPCR Calculators

  • Delta Delta Ct (2^-ΔΔCt) CalculatorCompute qPCR fold change with the Livak 2^-ΔΔCt method: enter target and reference Ct for test and control to get ΔCt, ΔΔCt, fold change and log2 FC.
  • PCR Master Mix CalculatorScale a PCR master mix for any number of reactions: per-tube and total µL of 2X mix, primers, dNTP, template and water, with spare-reaction overage.
  • Primer Tm CalculatorCalculate primer melting temperature (Tm) by SantaLucia 1998 nearest-neighbour or Wallace rule, with annealing temperature, Na⁺ salt correction and GC%.
  • qPCR Efficiency CalculatorCalculate qPCR amplification efficiency from a standard curve: regress Cq on log10 concentration for slope, R² and E = 10^(−1/slope) − 1, flagging 90–110%.

Cell Culture Calculators

Protein Analysis Calculators

Molecular Cloning Calculators

  • Ligation Ratio CalculatorCalculate the insert:vector mass for any molar ratio, or find the ratio you actually ligated — with the derivation, worked examples and MW cross-links.
  • Plasmid Molecular Weight CalculatorTurn a plasmid size in bp into molecular weight, pmol/µg, molarity and copy number for cloning — the workflow bundle plain DNA MW tools leave out.
  • Restriction Enzyme Site FinderFind every restriction enzyme that cuts your DNA sequence — scans the full REBASE set in your browser, with cut positions and fragment sizes.
  • Transformation Efficiency CalculatorConvert colony counts to transformation efficiency in CFU/µg, with the plated-volume dilution chain, typical ranges and links to the CFU calculator.

Biochemistry Calculators

  • Beer-Lambert Law CalculatorSolve A = ε·c·l for absorbance, concentration, molar extinction coefficient or path length, with dilution factor, mass output and reliability warnings.
  • Buffer CalculatorBuffer recipes from the charge-and-mass balance: grams to weigh, NaOH or HCl to add, the pH it reads back, capacity and co-ion load.
  • Michaelis-Menten CalculatorSolve the Michaelis-Menten equation for v, Vmax, Km or [S], with unit switching, kcat and catalytic efficiency readouts, and inhibitor classification.

Microbiology Calculators

  • OD600 CalculatorConvert OD600 to cells/mL and g DCW/L using per-organism factors from BioNumbers, with their published spread, dilution handling and self-calibration.

Genetics Calculators

  • Hardy-Weinberg CalculatorHardy-Weinberg calculator: allele and genotype frequencies from counts or disease rates, with the chi-square test, exact test, F and a 95% interval.
  • Punnett Square CalculatorPunnett square calculator for mono- and dihybrid crosses, plus carrier risk across children, X-linked and linked-gene crosses with exact probabilities.

Prefer the reasoning behind a number? The lab calculation guides walk each formula through by hand, one calculation per page.